产品说明
一般描述
The Human Shh-N (Sonic Hedgehog N Terminus) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme- linked immunosorbent assay for the quantitative measurement of human Shh-N in serum, plasma, cell culture supernatants and urine.
免疫原
Recombinant Human ShhN
应用
请参考Protocol了解详情。
生化/生理作用
Sonic hedgehog (Shh) is a secreted glycoprotein involved in embryonic morphogenic development of several tissues. It has a role in fracture healing and regeneration. In the canonical pathway, the protein associates with its receptor patched (Ptc-1), de-represses smoothened (Smo) and activates the Gli family of transcription factors. Mutations in the Shh pathway have been linked to developmental diseases and cancer. Shh plays a vital role in axon growth and guidance throughout the brain development. Shh stimulates neurite outgrowth by overcoming neurite growth inhibitory environment associated with glial scar.
其他说明
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
基本信息
| NACRES | NA.32 |
产品性质
| species reactivity | human |
| 包装 | kit of 96 wells (12 strips x 8 wells) |
| technique(s) | ELISA: suitable capture ELISA: suitable |
| input | sample type serum sample type cell culture supernatant(s) sample type urine sample type plasma |
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 42 pg/mL standard curve range: 40.96-10000 pg/mL (Assay Diluent C) inter-assay cv: <12% intra-assay cv: <10% sensitivity: 8 pg/mL standard curve range: 40.96-10000 pg/mL (Assay Diluent B) |
| detection method | colorimetric |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... SHH(6469) |
组份列表
组份可单独销售
| 货号 | 组份 |
| RABELADB | ELISA 5X Assay/Sample Diluent Buffer B (Item E1) |
| RABELADC | ELISA 1X Assay/Sample Diluent Buffer C (Item L) |
| RABSTOP3 | ELISA Stop Solution (Item I) |
| RABTMB3 | ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) |
| RABWASH4 | 20X Wash Buffer (Item B) |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H290 |
| 预防措施声明 | P234 - P390 |
| 危险分类 | Met. Corr. 1 |
| 储存分类代码 | 8A - Combustible, corrosive hazardous materials |
| WGK | WGK 3 |
Sigma-Aldrich
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