产品说明
一般描述
Lipolysis is the process of hydrolyzing triglycerides to free fatty acids and glycerol. This process involves the action of adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase. Lipolysis maintains the energy balance during fasting and exercise by providing a substrate for oxidative metabolism. Lipolysis is regulated by nutritional factors and hormones. Problems with the regulation of lipolysis are associated with obesity, diabetes, and metabolic syndromes.
适用性
该试剂盒适用于从组织中分离脂肪细胞和脂解作用的测定。
原理
The Lipolysis (Adipocyte) Kit provides contains synthetic catecholamine (isoproterenol) that activates β-adrenergic receptors. This results in the activation of adenylate cyclase that converts ATP to cAMP. cAMP then activates the hydrolysis of triglycerides by hormone-sensitive lipase. Lipolysis is determined by measuring a fluorescent (λex = 535/ λem = 587 nm) or colorimetric (570 nm) product proportional to the amount of glycerol present.
基本信息
| EC 号 | 259-322-7 |
| NACRES | NA.25 |
产品性质
| detection method | colorimetric fluorometric |
| 储存温度 | −20℃ |
组份列表
组份不可单独销售
| 货号 | 组份 |
| Collagenase (0.2%) | |
| Collagenase Stop Buffer | |
| Adipocyte Wash Buffer | |
| Adipocyte Lipolysis Buffer | |
| Glycerol Assay Buffer | |
| Glycerol Probe, in DMSO | |
| Glycerol Enzyme Mix | |
| Glycerol Standard, 100 mM | |
| Isoproterenol, 10 mM | |
| Cell Strainer |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H315 - H319 - H335 |
| 预防措施声明 | P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338 |
| 危险分类 | Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
Sigma-Aldrich
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