MCF10A CELLS PTEN (-/-) PIK3CA H1047R (+/-)

产品说明

一般描述

MCF10A CELLS PTEN (-/-) PIK3CA H1047R (+/-) are pre-neoplastic mammary epithelial cells from a human adult female with ZFN modifications.
This cell line was derived from ATCC Catalog No. CRL-10317.

应用

Media Renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37℃ in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:8
The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat. No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat. No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat. No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat. No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat. No. H6909, to a final concentration of 0.5 ug/mL.
Cell freezing medium - DMSO 1X, Cat. No. C6164.

特点和优势

These MCF10A cells are adherent with a doubling time of approx. 20 hours
Zinc finger nuclease (ZFN) modifications on chromosomes 10q23.3 and 3q26.3

质量

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

基本信息

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