MDR1 Knockout HepaRG^TM Cells

产品说明

一般描述

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG MDR1 knockout cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

应用

See technical bulletin for detailed protocols

特点和优势

Zinc finger nucleases (ZFN) mediated Knock-out of MDR1 gene.

• The frame-shift mutation of MDR1 gene was confirmed by fragment length analysis and DNA sequencing.
• Loss of functionality was confirmed by loss of transport of selective substrates in sandwich culture assay.

HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity.

• Sigma′s HepaRG MDR1 Knockout (KO) allows investigations of drug-transporter interactions involving MDR1 (P-glycoprotein) in the liver.

质量

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

法律信息

These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.
HepaRG is a trademark of BioPredic International company

基本信息

NACRES

NA.81

产品性质

质量水平	200
生物来源	human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)
形式	liquid
储存温度	−196℃
Gene Information	human ... ABCB1(5243)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable