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位置:首页 > 品牌 > Sigma-Aldrich > Monoclonal Anti-Alkaline Phosphatase, Human Placental-Agarose antibody produced in mouse

Monoclonal Anti-Alkaline Phosphatase, Human Placental-Agarose antibody produced in mouse

品牌
Sigma-Aldrich
货号
A2080
规格纯度
clone 8B6, purified immunoglobulin, PBS suspension
参考价格
5433.05 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Monoclonal Anti-Alkaline Phosphatase, Human Placental-Agarose antibody produced in mouse
PLAP
Anti-Phosphatase, Alkaline, Human Placental
Monoclonal Anti-Alkaline Phosphatase, Human Placental
产品介绍:

产品说明

一般描述

Monoclonal Anti-Human Placental Alkaline Phosphatase (mouse IgG2a isotype) is derived from the 8B6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Alkaline Phosphatase (AP) is a broad and general term associated with non-specific phosphomonoesterases, with optimal activity at alkaline pH. Human placental alkaline phosphatase (EC 3.1.3.1; hPLAP), an isoenzyme of the AP group of enzymes, is ordinarily synthesized in the placental syncytiotrophoblast, becoming detectable in maternal circulation after the twelfth week of pregnancy. Small amounts of hPLAP are also found in the endocervix, fallopian tubes and lung. Very small amounts of heat-stable AP resembling hPLAP (hPLAP-like AP) are expressed in the testis, thymus and in rare colon epithelial cells.
In SDS gels, the product reacts with both Regan and Nagao isozymes of human placental alkaline phosphatase (hPLAP, 130 kDa, 67/130 kDa). By RIA, the antibody binds to hPLAP with an affinity constant of 5 × 109 LM-1. It does not react with PLAP-like enzymes.

免疫原

Human epidermoid carcinoma cell line expressing placental alkaline phosphatase.

应用

Cultured pancreatic islet cells were fixed with cold 4% paraformaldhyde at room temperature for 10 minutes and incubated with mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody at 1:500. Mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody was used to detect a unique molecular probe that consisted of the FGFR extracellular domain expressed as a chimera with human PLAP.
Anti-Alkaline phosphatase human placental antibody coupled to agarose was packed in a column and used to purify alkaline-phosphatase conjugated FGF receptor out of conditioned media from COS-7 cells. Column was washed with 1M NaCl.
This immunoadsorbant may be used to purify fusion proteins produced using vectors expressing the hPLAP gene. Suitable for affinity purification and immunoprecipitation procedures of human placental alkaline phosphatase either natural or recombinant.It is alsu used in western blotting.

包装

1 mL in glass bottle

生化/生理作用

The human placental alkaline phosphatase (hPLAP and hPLAP-like AP) is present in the sera and tumor tissue of patients suffering from various types of cancer. Despite the continued interest in hPLAP as a potential tumor marker, it has not been widely used in the routine clinical laboratory, mainly because of the low overall specificity attained by methodologies such as heat-inactivation and sensitivity to L-phenylalanine.
Alkaline phosphatases are non-specific phosphomonoesterases that function optimally at alkaline pH. Placental alkaline phosphatase differs from the alkaline phosphatases of bone, liver and kidney, in the hydrolysis rate of various phosphate-based substrates, and also in its degree of inhibition by L-phenylalanine and L-homoarginine or levamisole. Human Placental Alkaline Phosphatase (hPLAP) has been studied as a potential tumor marker.

外形

Suspension in 0.1 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

制备说明

Prepared using purified antibody coupled to cyanogen bromide-activated agarose.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00162106
NACRESNA.46

产品性质

质量水平200
生物来源mouse
偶联物agarose conjugate
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆8B6, monoclonal
形式PBS suspension
species reactivityhuman
标记范围2 mg antibody per mL bed volume
technique(s)immunoprecipitation (IP): suitable
同位素/亚型IgG2a
UniProt登记号P05187
运输wet ice
储存温度2-8℃
Gene Informationhuman ... ALPP(250)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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