产品说明
一般描述
The Mouse Shh-N (Sonic Hedgehog N Terminus) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Shh-N in serum, plasma, cell culture supernatants and urine.
免疫原
Recombinant Mouse Sonic Hedgehog (N-terminus)
应用
For research use only. Not for use in diagnostic procedures.
Mouse Shh-N ELISA Kit has been used to measure serum sonic hedgehog (Shh) level.
生化/生理作用
Sonic hedgehog (Shh) is a secreted glycoprotein involved in embryonic morphogenic development of several tissues. It has a role in fracture healing and regeneration. In the canonical pathway, the protein associates with its receptor Patched (Ptc-1), de-represses Smoothened (Smo) and activates the Gli family of transcription factors. Mutations in the Shh pathway have been linked to developmental diseases and cancer. Shh plays a vital role in axon growth and guidance throughout the brain development. Shh stimulates neurite outgrowth by overcoming neurite growth inhibitory environment associated with glial scar.
其他说明
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
基本信息
NACRES | NA.32 |
产品性质
species reactivity | mouse |
包装 | kit of 96 wells (12 strips x 8 wells) |
technique(s) | ELISA: suitable capture ELISA: suitable |
input | sample type serum sample type cell culture supernatant(s) sample type plasma |
assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 5 pg/mL standard curve range: 4.1-1000 pg/mL |
detection method | colorimetric |
运输 | wet ice |
储存温度 | −20℃ |
Gene Information | mouse ... Shh(20423) |
组份列表
组份可单独销售
货号 | 组份 |
RABELADA | ELISA 1X Assay/Sample Diluent Buffer A (Item D1) |
RABELADB | ELISA 5X Assay/Sample Diluent Buffer B (Item E1) |
RABSTOP3 | ELISA Stop Solution (Item I) |
RABTMB3 | ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) |
RABWASH4 | 20X Wash Buffer (Item B) |
安全信息
象形图 | |
警示用语: | Warning |
危险声明 | H290 |
预防措施声明 | P234 - P390 |
危险分类 | Met. Corr. 1 |
储存分类代码 | 8A - Combustible, corrosive hazardous materials |
WGK | WGK 3 |
Sigma-Aldrich