MRP3 Knockout Caco-2 Cells

产品名称：

产品介绍：

产品说明

一般描述

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MRP3 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCC3 (MRP3) efflux transporter.

应用

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

特点和优势

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:

A functional knockout of the MRP3 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP3 substrate
The vial format enables the MRP3 knockout cells to be included in standard drug transporter protocols
Human assay with no interference from animal inhibitors
Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

法律信息

ADME/Tox Cell Lines License

基本信息

NACRES

NA.81

产品性质

质量水平	200
生物来源	human male colorectal tissue (Source Disease: colon adenocarcinoma)
形式	liquid
technique(s)	drug transporter assay: suitable permeability assay: suitable
application(s)	ADME/TOX
储存温度	−196℃
Gene Information	human ... ABCC3(8714)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable