产品说明

一般描述

髓过氧化物酶(MPO：EC1.11.2.2)是一种在中性粒细胞中大量表达的过氧化物酶。它能够催化卤化物离子的过氧化氢依赖性氧化反应并生成次氯酸盐(HClO)，MPO通过该反应对肿瘤细胞和微生物发挥细胞毒活性。MPO还能够氧化苯酚和苯胺等多种物质。MPO对氯化或过氧化反应的催化作用，取决于氯化物和还原底物的相对浓度。最近的研究表明，MPO水平升高与心血管疾病和心肌梗塞风险增加相关，因此，开发MPO的新型和特异性抑制剂对于疾病治疗至关重要。

特点和优势

Compatible with high-throughput handling systems.

适用性

Suitable for screening/characterizing/studying potential inhibitors of Myeloperoxidase

原理

This MPO Inhibitor Screening Kit provides screening assays for both MPO chlorination and peroxidation activities. In the chlorination assay (specific for MPO), MPO converts hydrogen peroxide and sodium chloride to sodium hypochlorite, which reacts with the chlorination substrate to give an intensely fluorescent product, ex = 480 nm/em = 520. In the peroxidation assay, MPO oxidizes peroxidation substrate to generate fluorescence ex = 535 nm/em = 587. The fluorescence generated is directly proportional to any peroxidase activity present. In the presence of MPO inhibitor, reactions are impeded, thus decreasing the rate and/or extent of generation of MPO-dependent fluorescence.

产品性质

安全信息