Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric)

产品说明

一般描述

Phosphoglycerate Dehydrogenase (PHGDH) is the first enzyme that participates in the L-serine synthesis pathway. PHGDH catalyzes the reversible reaction that converts 3-phosphoglycerate into 3 phosphohydroxyglycerate and the NADH-dependent reduction of a-ketoglutarate into D-2-hydroxyglutarate (D-2HG). In humans, PHGDH deficiency causes severe health problems including congenital microcephaly, psychomotor retardation, and intractable seizure. Recent studies have shown increased PHGDH activity in a wide variety of cancers, particularly in breast and melanoma tumors. Therefore, the measurement of PHGDH activity has become of great importance in searching potential therapeutic agents against cancer.

特点和优势

Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit:

• Compatible with high-throughput handling systems.
• Includes Positive Control.
• Sensitive, detecting phosphoglycerate dehydrogenase activity less than 0.1 mU per sample.

适用性

The kit is suitable for the measurement of PHGDH activity in various tissues (liver and kidney, etc.) and cells (adherent or suspension cells) and for the analysis of de novo L-serine biosynthesis.

原理

The Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay kit provides a quick and easy method for monitoring PHGDH activity in a variety of samples. In this kit, phosphoglycerate dehydrogenase converts 3-phosphoglycerate and NAD into 3-phosphohydroxyglerate and NADH respectively. The oxidation of NADH reduces a probe generating a strong, stable absorbance signal at A₄₅₀ nm.

产品性质

安全信息

象形图
警示用语：	Danger
危险声明	H314 - H335
预防措施声明	P261 - P271 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P305 + P351 + P338
危险分类	Eye Dam. 1 - Skin Corr. 1B - STOT SE 3
靶器官	Respiratory system
储存分类代码	8A - Combustible, corrosive hazardous materials