ReadyShield^® Protease Inhibitor Cocktail

产品说明

一般描述

This particular protease inhibitor cocktail omits chelators (e.g., EDTA), to optimize its use for purification of histidine-tagged proteins.1 It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. The cocktail has been tested on extracts from E. coli and insect cells This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0004 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P8849.

特点和优势

• Does not freeze in storage for quick, convenient use.
• Tailored for use with Histidine fusion tagged proteins.
• Suggested as 100 X solution.
• Reclosable screw-top vials.
• Can be stored in cooler for approx. 1 month or room temperature for a week.

制备说明

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 100 ml of lysate from 20 g (wet weight) of E. coli cells or 10 g (wet weight) of baculovirus-infected insect (Spodoptera frugiperda) cells.

其他说明

For life science research only. Not for use in diagnostic procedures.

法律信息

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息