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Anti-α-Actinin antibody produced in rabbit

品牌
Sigma-Aldrich
货号
A2543
规格纯度
whole antiserum
参考价格
5149.17 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-α-Actinin antibody produced in rabbit
产品介绍:

产品说明

一般描述

α-Actinin is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. Rabbit anti-α-actinin antibody stains α-actinin in cell cultures using indirect immunofluorescence. The antibody reacts best with cultured chicken fibroblasts, however, adequate labeling may also be obtained with cells of other species. In an immunoblot assay, the antiserum specifically stains the α-actinin band.
α-Actinin is a member of the spectrin superfamily. It consists of an antiparallel homodimer, with an N-terminal actin-binding domain (ABD), a central domain of four spectrin-like repeats (SRs), and a C-terminal calmodulin-like domain (CAMD) with two pairs of EF-hand motifs (EFs). α-Actinin exists in four isoforms: α-actinin 1, α-actinin 2, α-actinin 3 and α-actinin 4. In smooth muscles, α-actinin is present in dense bodies and plaques whereas, in normal skeletal muscles, it is associated with Z-discs that define muscle sarcomere.

特异性

Anti-α-Actinin specifically stains α-actinin in cell cultures using indirect immunfluorescent labeling techniques. The antibody reacts best with cultured chicken fibroblasts, however adequate labeling may also be obtained with cells of other species.

免疫原

α-actinin from chicken gizzard

应用

Rabbit anti-α-actinin antibody has been used for:

  • co-immunoprecipitation studies
  • immunoblotting studies
  • immunofluorescence

Anti-Actinin antibody was used to detect actinin in human mesangial cells. Cells were first grown on glass cover slips, fixed in cold methanol and permeabilized with 3.5% paraformaldehyde/0.1% Tween-20 for 5 min at room temperature.

生化/生理作用

α-Actinin is an actin-crosslinking Z-disk protein. It plays a vital role in linking the cytoskeleton to different transmembrane proteins in a variety of junctions. α-Actinin modulates receptor activities and serves as a cytoskeleton scaffold for diverse signaling pathways. It is implicated in muscle contraction.

外形

Supplied as a liquid with 0.1% sodium azide as a preservative.

储存及稳定性

For continuous use, store at 2-8 ℃ for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00162107
NACRESNA.41

产品性质

生物来源rabbit
质量水平200
偶联物unconjugated
抗体形式whole antiserum
antibody product typeprimary antibodies
克隆polyclonal
包含15 mM sodium azide
species reactivitychicken
technique(s)indirect immunofluorescence: 1:500 using cultured chicken fibroblasts
western blot: suitable using cultured cells and chicken gizzard extract
UniProt登记号O43707
P35609
运输dry ice
储存温度−20℃
Gene Informationchicken ... ACTN2(396263), ACTN4(396024)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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