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位置:首页 > 品牌 > Sigma-Aldrich > CRISPR-Lenti Human HPRT1 Positive Control Transduction Particles

CRISPR-Lenti Human HPRT1 Positive Control Transduction Particles

品牌
Sigma-Aldrich
货号
CRISPR13V
包装型号
规格纯度
参考价格
3683.8 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
CRISPR-Lenti Human HPRT1 Positive Control Transduction Particles
产品介绍:

产品说明

一般描述

The Sigma CRISPR-LENTI HUMAN HPRT1 POSITIVE CONTROL TRANSDUCTION PARTICLES are a critical positive control to monitor transduction efficiency for lentiCRISPRs. This control is produced from the sequence-verified CRISPR lentiviral plasmid targeting human HPRT1. The protein encoded by this gene is a transferase which plays a central role in the generation of purine nucleotides. Cells with intact HPRT1 will die upon exposure to 6-TG (A4882), an antimetabolite used in the treatment of leukemias. Conversely, cells with HPRT1 knocked out will increase over time with 6-TG selection. This targets a validated HPRT1 CRISPR site, which serves as an experimental control for Sigma lenti CRISPRs. The Sigma lenti CRISPR positive control is a one-component system consisting of an EF1-alpha- driven Cas9, a U6-driven guide RNA plasmid targeting the human HPRT1 gene, with both Puromycin and GFP co-expressed with Cas9.
Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.

应用

Functional Genomics/Screening/Target Validation

  • Positive control, HPRT1, CRISPR lentivirus format, for creating gene knockouts/genetic modifications in cell lines.

To see more application data, protocols, vector maps visit sigma.com/crispr.

特点和优势

Serves as a positive experimental control for the CRISPR editing workflow using lentiCRISPRs (virus format). Allows for validation of your system with the lentiCRISPR/Cas9 system. A positive result in a mis-match detection assay will indicate validation of your system.

外形

200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

法律信息

CRISPR专利正在申请中

基本信息

NACRESNA.51

产品性质

质量水平200
包装vial of 200 μL
浓度1x106  VP/ml (via p24 assay)
application(s)CRISPR
运输dry ice
储存温度−70℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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