产品说明
一般描述
Penicillinase from Bacillus cereus is both an extracellular and cell-bound enzyme. It has a single polypeptide chain and has a molecular weight of 31 kDa. It lacks cysteine in the protein sequence.
应用
用于生产青霉素。
Penicillinase from Bacillus cereus may be used in the preparation of biosensor for amperometric detection of hydrolysis of penicillin.
生化/生理作用
青霉素酶特异性催化青霉素的 β-内酰胺环的水解。
包装
Bottomless glass bottle. Contents are inside inserted fused cone.
单位定义
一个单元将在 pH7.0,25℃ 下,每分钟水解 1.0 μmole 指定的底物。以苄青霉素单位出售。该国际单位(使用苄青霉素作为底物)约等于 600 Levy 或 75 Pollock 单位。
其他说明
Used to destroy β-lactam antibiotics in body fluids or culture media. For the determination of penicillin; In the coupled enzyme assay for isopenicillin N synthetase; ELISA of estradiol; Penicillinase optodes
基本信息
酶学委员会(EC)编号 | 3.5.2.6 ( BRENDA | IUBMB ) |
MDL编号 | MFCD00131819 |
NACRES | NA.54 |
产品性质
形式 | powder |
质量水平 | 100 |
质量 | lyophilized |
specific activity | ~13 U/mg |
分子量 | Mr ~30000 |
颜色 | white |
异质活性 | β-lactamase II ≤2% |
储存温度 | −20℃ |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H317 - H334 |
预防措施声明 | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
危险分类 | Resp. Sens. 1 - Skin Sens. 1 |
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
Sigma-Aldrich