产品说明
应用
MTT Formazan has been used in cell viability assay.
生化/生理作用
MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.
其他说明
评审:四唑盐和甲瓒
基本信息
经验(实验)分子式 | C18H17N5S |
分子量 | 335.43 |
MDL编号 | MFCD00080731 |
PubChem化学物质编号 | 329769650 |
NACRES | NA.83 |
产品性质
质量水平 | 100 |
形式 | powder |
溶解性 | chloroform: 10 mg/mL, clear, dark violet |
SMILES string | Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C |
InChI | 1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+ |
InChI key | GIFKVQOPAJDJLO-SZVLODPRSA-N |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich