产品说明
一般描述
RNase A(核糖核酸酶A)是一种内切核糖核酸酶，在嘧啶核苷酸后裂解单链RNA的磷酸二酯键。它可切割3′磷酸基末端(例如，pG-pG-pC-pA-pG将切割为pG-pG-pCp 和A-pG)。对单链RNA表现出最高活性。RNase A是含有四个二硫键的单链多肽。它与RNase B不同，并非糖蛋白。核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNase A还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。RNAse在重金属离子存在时受到抑制。此外，RNase也被DNA竞争性抑制。
Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds. The ribonuclease (RNase) gene is mapped to human chromosome 14.
应用
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the mechanism of heavy metal ions on RNase activity. Ribonuclease A from bovine pancreas has also been used in a study to investigate the performance of oligomeric poly(diallyldimethylammonium chloride) as displacer for cation-exchange displacement chromatography of proteins.
生化/生理作用
The structure of Ribonuclease A (RNase A) comprises of four disulfide and catalytic triad of two histidines crucial for its functionality. RNase A displays three-dimensional domain swapping of the α-helical N-terminal and the C-terminal β-strand. It can exist as dimer or oligomers. The level of RNase is elevated in cancers and infectious diseases. RNase A family of enzymes serve as potential drug targets. The homolog and variants of RNase A are potential chemotherapeutic agents.
特点和优势
我们高度稳定的核糖核酸酶A——RNase A，适合于RNA去除、RNA测序和DNA纯化。
外形
0.2M 磷酸钠缓冲溶液，pH 6.4
制备说明
R5503 的进一步色谱纯化得到的特纯 RNAse A
分析说明
蛋白测定方法：E.
通过 SDS-PAGE 测定 RNase A 纯度
基本信息
| 酶学委员会(EC)编号 | 3.1.27.5 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD00132181 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 生物来源 | bovine pancreas |
| 类型 | Type X-A |
| 测定 | ≥90% (SDS-PAGE) |
| 形式 | buffered aqueous solution |
| specific activity | ≥70 Kunitz units/mg protein |
| 分子量 | ~13,700 |
| 异质活性 | protease, essentially free |
| 储存温度 | −20℃ |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H334 |
| 预防措施声明 | P261 - P284 - P501 |
| 危险分类 | Resp. Sens. 1 |
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves |
Sigma-Aldrich
m.cnreagent.com
