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Transcreener® EPIGEN Methyltransferase Assay

品牌
Sigma-Aldrich
货号
3017
包装型号
规格纯度
参考价格
18172.7 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Transcreener® EPIGEN Methyltransferase Assay
产品介绍:

产品说明

一般描述

The Transcreener® EPIGEN Methyltransferase Assay is a universal biochemical HTS assay for enzymes that produce S-adenosylhomocysteine (SAH), including all enzymes within the histone (HMTs) and DNA (DNMTs) methyltransferase families. It combines the extensively validated Transcreener® AMP2/GMP2 Assay, which relies on fluorescent immunodetection of AMP, with coupling enzymes that convert SAH to AMP. Enzyme activity is signaled by a decrease in fluorescence polarization as the bound tracer is displaced from the Transcreener® AMP2/GMP2 Antibody. The assay is a simple mix-and-read format with two liquid addition steps. Methyltransferase (MT) enzyme reactions are first quenched with Stop Buffer and then the SAH Detection Mixture containing coupling enzymes, Transcreener® AMP2/GMP2 antibody, and tracer is added. The assay provides excellent signal at low substrate conversion, with a Z′ =0.7 and =100 millipolarization shift (mP) under normal reaction conditions.

View full Transcreener® product list

数量

3017-1K = 1,000 assay, 384-well
3017-10K = 10,000 assay, 384-well

外形

Kit with buffered aqueous solutions

法律信息

Transcreener is a registered trademark of BellBrook Labs

基本信息

NACRESNA.84

产品性质

运输dry ice
储存温度−20℃

Sigma-Aldrich

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