产品说明
应用
Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.
生化/生理作用
Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.
单位定义
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 ℃
重悬
The solution can be used without dialysis (for enzyme immuno-assay).
其他说明
Sales restrictions may apply
Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase
基本信息
| 酶学委员会(EC)编号 | 3.1.3.1 ( BRENDA | IUBMB ) |
| EC 号 | 232-631-4 |
| MDL编号 | MFCD00131849 |
| eCl@ss | 42010105 |
| NACRES | NA.54 |
产品性质
| 形式 | solution (clear, colorless) |
| specific activity | ~2500 U/mg protein (~10 mg/ml) |
| 分子量 | Mr ~140000 |
| technique(s) | enzyme immunoassay: suitable |
| 杂质 | 0.1 mM ZnCl2 1 mM MgCl2 3 M NaCl 30 mM triethanolamine |
| pH值(酸碱度) | ~7.6 |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich
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