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Transcreener® GDP FP Assay

品牌
Sigma-Aldrich
货号
3009
包装型号
规格纯度
参考价格
11446.9 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Transcreener® GDP FP Assay
产品介绍:

产品说明

一般描述

The Transcreener® HTS Assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener® HTS Assay platform eliminates delays involved in assay development for new HTS targets, and greatly simplifies compound and inhibitor profiling across multiple target families.

The Transcreener® GDP Assay is a far-red, competitive fluorescence polarization (FP) assay based on the detection of GDP and therefore is compatible with any enzyme that produces GDP, including GTPases such as CDC42. The Transcreener® GDP Assay is a simple one step homogenous detection assay, and is extremely flexible with regard to GTP concentration (1 to 1,000 μM GTP). The assay provides excellent signal at low substrate conversion, with a Z′ = 0.7 and =85 polarization shift (mP) at 10% GTP conversion using 10 μM GTP.

The Transcreener® GDP Assay was developed to follow the progress of any enzyme that produces GDP. The Transcreener® GDP Detection Mixture comprises a GDP Alexa633 Tracer bound to a GDP Antibody. The tracer is displaced by GDP, the invariant product generated during the enzyme reaction. The displaced tracer freely rotates leading to a decrease in fluorescence polarization. Therefore, GDP production is proportional to a decrease in polarization. The assay uses a far red tracer to minimize interference from fluorescent compounds and light scattering.

View full Transcreener® product list

数量

3009-1K = 1,000 assay, 384-well
3009-10K = 10,000 assay, 384-well

外形

Kit with buffered aqueous solutions

法律信息

Transcreener is a registered trademark of BellBrook Labs

基本信息

NACRESNA.84

产品性质

运输dry ice
储存温度−20℃

Sigma-Aldrich

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