FLUO 3/AM-CAS 121714-22-5-Calbiochem

产品说明

一般描述

Membrane-permeable version of FLUO 3 (Cat. No. 343244). Cleavage of FLUO 3/AM by cytosolic esterases traps the indicator in the cell. FLUO 3 is almost nonfluorescent at resting calcium levels, but fluorescence levels increase 40-fold upon calcium binding. Unlike all other Ca²⁺ indicators, FLUO 3/AM is nonfluorescent until it is hydrolyzed by the cell. Since its excitation maximum is at 506 nm, this compound can be used in conjunction with caged compounds, which are released by UV light. Its relatively high K_d for Ca²⁺ facilitates the measurement of higher peaks of Ca²⁺ transients than is possible with FURA 2. FLUO 3 has an excitation maximum of 506 nm and an emission maximum of 526 nm in the presence of high and low calcium concentrations.
Membrane-permeable version of FLUO 3 (Cat. No. 343244). Unlike all other Ca²⁺ indicators, FLUO 3/AM is non-fluorescent until it is hydrolyzed in the cell by cellular esterases. It is insensitive to Mg²⁺.

生化/生理作用

Primary Target
Ca2+ indicators

警告

Toxicity: Standard Handling (A)

其他说明

Caputo, C., and Bolanos, P. 1994. J. Physiol. (London)481, 119.
Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134.
Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun.163, 309.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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