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位置:首页 > 品牌 > Sigma-Aldrich > CRISPR dCas9-p300 RFP Activator Expression Plasmid

CRISPR dCas9-p300 RFP Activator Expression Plasmid

品牌
Sigma-Aldrich
货号
DCAS9P300RFP
包装型号
规格纯度
参考价格
2486 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
CRISPR dCas9-p300 RFP Activator Expression Plasmid
产品介绍:

产品说明

一般描述

This gene activation system is based on a fusion of inactive Cas9 (dCas9) to the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300. The dCas9-p300 activator expression plasmids use the CMV promoter for strong transient expression of dCas9-P300 and RFP linked by a 2A peptide (CMV-dCas9-p300-2A-RFP) allowing for easy visualization of successful transfection. The dCas9-P300-RFP expression plasmid is one part of a two part CRISPR system with individual dCas9-P300 and gRNA expression vectors.

To order gRNA in any format click here

应用

Functional Genomics/Target Validation

  • Epigenetic Modification
  • Transcriptional Activation
  • Manufacture of dCas9-P300 expressing lentiviral particles

特点和优势

The Sigma CRISPR dCas9p300 plasmid co-expresses p300-HAT and RFP, to easily monitor delivery and expression in your target cell type. gRNAs can successfully direct nuclease-deficient Cas9 (dCas9) fused to p300 HAT catalytic domain to increase levels of histone acetylation and endogenous gene expression. The dCas9-p300 histone acetylation approach represents a distinct mechanism of action relative to dCas9-VP64 or other similar gene activation motifs.

原理

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). Mutations to the catalystic domains, RuvC and HnH, render it inactive as a nuclease yet still allow for the protein to be programmed with a single gRNA, directing p300 epigenetic modification cargo to any desired genomic location.

法律信息

CRISPR Use License Agreement

Evrogen Fluorophore Licenses

产品性质

质量水平200
重组expressed in E. coli
包装vial of 50 μL
浓度20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
application(s)CRISPR
运输dry ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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