产品说明

一般描述

Human IgMs are the initial immunoglobulin isotypes that appear in blood in response to the first exposure to external antigens. These immunoglobulins have been implicated in CNS myelin repair. Increased levels of secretory IgM have been linked to rhino-conjunctivitis . Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is specific for human IgM when tested against human IgA, IgG, IgM, Bence Jones κ, and λ myeloma proteins.
Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection.
Alkaline Phosphatase is an enzyme that catalyzes the conversion of chromogenic substrates such as p-nitrophenylphosphate (PNPP); chemiluminescent substrates such as CDP-Star^® and fluorogenic substrates such as 4-methylumbelliferyl phosphate (4-MUP) into detectable chromophores, light-emitters or fluorescers, respectively.

免疫原

Human IgM

应用

Goat polyclonal anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody may be used to detect and quantitate the level of IgM in human serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques.
Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is suitable for use in ELISA.
IgM is a glycoprotein with 5 n-linked glycosylation sites on the heavy chain. An ELISA assay was performed to identify glycosylated forms of IgM that bind to lectin. Alkaline phosphatase conjugated goat anti-human IgM was used as the secondary at 1:2000 and developed using p-nitrophenyl substrate (Sigma).
Goat Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody can be used for dot blot and western blot applications at 1:30,000.

外形

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

法律信息

CDP-Star is a registered trademark of Tropix, Inc.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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