产品说明
一般描述
IgM antibody is produced by B cells and has pentamer structure which helps the antibody in polyreactivity and can also remove apoptotic cells. Anti-human IgM (μ-chain specific) -alkaline phosphatase antibody can be used in solid phase ELISA for determination of anti-sp75 antibodies. Goat anti human IgM-alkaline phosphatase antibody reacts specifically with human IgM.
免疫原
Human IgM.
应用
IgM is a glycoprotein with 5 n-linked glycosylation sites on the heavy chain. An ELISA assay was performed to identify glycosylated forms of IgM that bind to lectin. Alkaline phosphatase conjugated goat anti-human IgM was used as the secondary at 1:2000 and developed using p-nitrophenyl substrate (Sigma).
It may be used for immunoblotting.
外形
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 15 mM sodium azide and 50% glycerol
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
MDL编号 | MFCD00162460 |
NACRES | NA.46 |
产品性质
质量水平 | 200 |
生物来源 | goat |
偶联物 | alkaline phosphatase conjugate |
抗体形式 | affinity isolated antibody |
antibody product type | secondary antibodies |
克隆 | polyclonal |
形式 | buffered aqueous glycerol solution |
species reactivity | human |
technique(s) | direct ELISA: 1:7,000-1:21,000 |
运输 | wet ice |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich