Anti-Peroxidase antibody produced in rabbit

产品说明

一般描述

Antiserum is determined to be immunospecific for horseradish peroxidase and does not inhibit the enzymatic activity of the peroxidase.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. If necessary, the fractionated antiserum is adsorbed using solid phase techniques. Antiserum is determined to be immunospecific by immunoelectrophoresis (IEP), against purified and crude horseradish peroxidase
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.
Peroxidase is a hemoprotein.

免疫原

Purified peroxidase from horseradish

应用

Anti-Peroxidase antibody produced in rabbit has been used in:

• immunoblot assays
• enzyme-linked-immunosorbent assay (ELISA)
• immunoprecipitation

生化/生理作用

Peroxidase helps in catalyzing the oxidation of a number of substrates with the help of hydrogen peroxide.

外形

由 0.01M 的磷酸缓冲盐溶液 (pH 7.2) 冻干

重悬

Reconstitute with deionized water.

分析说明

Specificity is determined by immunoelectrophoresis using a crude peroxidase extract.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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