产品说明
一般描述
Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.
应用
Suitable for denaturing dsDNA before transfer to membranes during Southern procedures.
组分
The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.
其他说明
0.5 N NaOH, 1.5 M NaCl
基本信息
| NACRES | NA.25 |
产品性质
| 质量水平 | 200 |
| 等级 | for molecular biology |
| 形式 | solution |
| 储存温度 | room temp |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H290 - H314 |
| 预防措施声明 | P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 |
| 危险分类 | Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B |
| 储存分类代码 | 8B - Non-combustible, corrosive hazardous materials |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
