产品说明
应用
Enhanced Avian Reverse Transcriptase可将RNA转录为DNA，同时显著提升mRNA模板合成cDNA的效率。这是由于本款增强型AMV-RT能够逆转录长链mRNA模板，能够有效穿越复杂的二级结构，可以通过RT-PCR反应检测低丰度mRNA。
生化/生理作用
逆转录酶能够加速dNTP形成磷酸二酯键，从而催化RNA模板整合到互补DNA中。
特点和优势
包装
提供1管10x反应缓冲液。
单位定义
一单位酶能够以多聚腺苷酸为模板，以12-18的oligo(dT)为引物，在10分钟内将1纳摩TMP整合进TCA沉淀材料中。
法律信息
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
eAMV is a trademark of sigma-aldrich Co. LLC
基本信息
| MDL编号 | MFCD01634820 |
| NACRES | NA.55 |
产品性质
| 质量水平 | 200 |
| 形式 | crystalline powder |
| 用途 | sufficient for 50 reactions |
| 特点 | dNTPs included: no hotstart: no |
| 浓度 | 20 units/μL |
| technique(s) | RT-PCR: suitable |
| 颜色 | colorless |
| input | purified RNA |
| 运输 | wet ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
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