产品说明
一般描述
The HA tag is a 9-amino acid tag, with the sequence YPYDVPDYA (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C), derived from the hemagglutinin (HA) protein at amino acid residues 98-106 in the HA sequence. The HA tag contains a high proportion of charged amino acid residues, which makes the HA tag likely to form a strong antibody recognition site. Monoclonal Anti-HA Agarose Conjugate recognizes native as well as denatured/reduced forms of HA-tagged proteins. This product is reactive with N-or C-terminal HA-tagged fusion proteins that are expressed in E. coli or in mammalian cells.
特异性
该抗体可识别HA-标签蛋白的天然和还原变性形式,并且可与 大肠杆菌 或哺乳动物表达的N-末端或C-末端HA-标签融合蛋白发生反应。
免疫原
与人流感病毒血凝素(HA)片段相对应的合成肽,称为HA-标签,与KLH结合
应用
适用于免疫沉淀检测以及HA标记融合蛋白的免疫亲和纯化。
外形
悬浮于含有15 mM叠氮化钠的0.01 M磷酸盐缓冲液中
制备说明
Prepared using cyanogen bromide-activated agarose. Specific antibody concentration is 2.0 - 2.4 mg/mL settled resin.
基本信息
MDL编号 | MFCD00803873 |
NACRES | NA.56 |
产品性质
质量水平 | 200 |
生物来源 | mouse |
偶联物 | agarose conjugate |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | HA-7, monoclonal |
形式 | PBS suspension |
同位素/亚型 | IgG1 |
容量 | 20-50 nmol/mL elution capacity (HA-tagged fusion protein) 30-50 nmol/mL binding capacity (HA-tagged fusion protein) |
运输 | wet ice |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich