产品说明

一般描述

Lipases hydrolyze ester bonds of triacylglycerols and in some conditions, catalyze the synthesis of ester bonds by transesterification. Lipase from Rhizopus niveus exists as a single polypeptide chain with a molecular mass of about 30-34kDa.

应用

Lipase from Rhizopus niveus has been used to determine the positional distribution of fatty acids within triacylglycerol by hydrolysis of the acyl ester linkage at C-1 and C-3. It has also been used to induce intragastric lipolysis and study its effects on felodipine (a medication) release from a hydrophilic, extended release tablet.

生化/生理作用

Lipase from Rhizopus niveus is specifically used to produce cocoa butter substitutes.
可水解甘油三酯、甘油二酯、甘油单酯(速率依次递减)。

单位定义

1U 相当于在 pH 7.7 和 40℃ 下每分钟从甘油三酯转化出 1μmol 脂肪酸所需的酶量(以橄榄油为底物)；如上所述的 300U 相当于在 pH 8.0 和 40℃ 下，以 Fluka^® 编号 62314 的甘油三油酸酯作为底物时的 ~1U。

其他说明

说明：以三乙酰甘油酯作为底物时，pH 为 7.4。孵化期：60 分钟。
底物特异性：优先水解 1- 和 3-位(长链脂肪酸的中部)；最佳 pH 5.0-7.0；最适温度 30-45℃；酶法选择性去除保护官能团(C 端 COOH 官能团)

基本信息

产品性质

安全信息