产品说明
一般描述
Monoclonal ANTI-FLAG M2 is a purified immunoglobulin, IgG1, monoclonal antibody, purified from culture supernatant of hybridoma cells, that binds to FLAG® fusion proteins. Unlike ANTI-FLAG M1 antibody, the M2 antibody will recognize the FLAG sequence at the N-terminus, Met-N-terminus, C-terminus, or at an internal site of FLAG fusion proteins. Monoclonal ANTI-FLAG M2 is useful for identification and capture of FLAG fusion proteins by common immunological procedures such as Western blots and immunoprecipitation. It is also useful for affinity purification of FLAG fusion proteins when bound to a solid support.
form: solution pH 7.4, containing 15 mM sodium azide
concentration: 3.0-5.0 mg/mL
应用
IB, IF, IP, FACS, ELISA
Antibody is recommended for use in several applications such as immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, and ELISA.
包装
polypropylene screw cap vial
制备说明
Dilute the antibody solution from 0.5-10 ug/mL in specified buffer
法律信息
ANTI-FLAG is a registered trademark of sigma-aldrich Co. LLC
FLAG is a registered trademark of sigma-aldrich Co. LLC
产品性质
生物来源 | mouse |
抗体形式 | purified immunoglobulin (purified IgG1 subclass) |
克隆 | M2, monoclonal |
保质期 | 4 yr |
纯化方式 | using Protein A |
储存温度 | −20℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich