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Pyrophosphate reagent

品牌
Sigma-Aldrich
货号
P7275
规格纯度
spectrophotometric assay
参考价格
1072.26 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Pyrophosphate reagent
产品介绍:

产品说明

应用

The product has been used for the measurement of pyrophosphate content of plant tissues. It has also been used as a rapid, routine check of pyrophosphate content by monitoring NADH oxidation in Solanum tuberosum tuber extracts.

生化/生理作用

The pyrophosphate detection kit couples the production of pyrophosphate to the consumption of NADH.

包装

A technical bulletin is included.

组分

ComponentConcentration
Imidazole × HCl, pH 7.4 45 mM
Citrate 5 mM
EDTA 0.10 mM
Mg2+, Mn2+, Co2+ 2 mM, 0.2 mM, 0.02 mM
β-NADH 0.8 mM
F-6-P 12 mM
Bovine Serum Albumin 5 mg/mL
Sugar Stabilizer 5 mg/mL
PPi-PFK 0.5 units/mL
Aldolase 7.5 units/mL
GDH 5 units/mL
TPI 50 units/mL

质量

A coupled enzyme system containing fructose-6-phosphate kinase (pyrophosphate-dependent), aldolase, triosephosphate isomerase, and α-glycerophosphate dehydrogenase.

原理

Two moles of NADH are oxidized to NAD+ per mole of pyrophosphate consumed. The reaction is monitored spectrophotometrically at 340 nm.
The reagent can be used to detect 10-100 nmoles of pyrophosphate.

制备说明

Dissolve the contents of one vial of Pyrophosphate Reagent in 4.0 mL of water.

基本信息

NACRESNA.54

产品性质

质量水平200
形式powder
用途1 vial sufficient for ≤4 assays
储存温度−20℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, type N95 (US)

Sigma-Aldrich

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