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Betaine solution

甜菜碱 溶液

品牌
Sigma-Aldrich
CAS
107-43-7
货号
B0300
规格纯度
5 M, PCR Reagent
参考价格
446.08 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
107-43-7
Betaine solution
甜菜碱 溶液
产品介绍:

产品说明

一般描述

Betaine also called trimethylglycine or N,N,N triethylammonium acetate, is an analog of glycine with three methyl groups. It is highly compatible with polymerase chain reaction (PCR) buffer mixture. Betaine is a PCR enhancing reagent that is widely used for improving the yield and specificity of PCR products, especially during the PCR amplification of targets rich in GC content or those that form secondary structures resulting in poor yield. Betaine facilitates DNA strand separation and manages the DNA melting temperature (Tm) difference between the GC and AT pairs in DNA. It stabilizes the ds DNA by equalizing the contribution of GC- and AT-base pairs. Betaine has been broadly used to optimize multiplex and ‘long and accurate′ polymerase chain reaction (LA-PCR). The addition of 1.0-1.7 M aqueous betaine to a PCR mixture has been reported to reduce the base pair composition dependence on DNA strand melting.

应用

甜菜碱溶液已被用作以下组分

  • 环介导等温扩增(LAMP)反应
  • 用于基因组DNA扩增的聚合酶链式反应
  • 用于端粒长度分析的定量聚合酶链式反应(qPCR)
  • PCR扩增基因组DNA之中的CGG重复片片段。

包装

每管1.5mL

基本信息

线性分子式(CH3)3N+CH2COO
分子量117.15
Beilstein3537113
MDL编号MFCD00012123
PubChem化学物质编号24891511

产品性质

质量水平300
等级PCR Reagent
形式liquid
包装vial of 1.5 mL
浓度5 M
technique(s)PCR: suitable
颜色 colorless
application(s)agriculture
异质活性DNase, RNase, none detected
储存温度2-8℃
SMILES stringC[N+](C)(C)CC([O-])=O
InChI1S/C5H11NO2/c1-6(2,3)4-5(7)8/h4H2,1-3H3
InChI keyKWIUHFFTVRNATP-UHFFFAOYSA-N

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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