产品说明
一般描述
This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.
应用
Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco¢s Modified Eagle¢s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove¢s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.
联系
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法律信息
AQmedia is a trademark of sigma-aldrich Co. LLC
产品性质
质量水平 | 400 |
无菌性 | sterile-filtered sterile |
产品线 | AQmedia™ |
形式 | liquid |
technique(s) | cell culture | mammalian: suitable |
杂质 | Endotoxin, tested |
组分 | glucose: yes NaHCO3: yes stable glutamine: yes phenol red: yes |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich