产品说明
应用
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.
数量
按照每升培养基16.4克粉末配制。
重悬
补充2.0 g/L碳酸氢钠。
法律信息
Hybri-Max is a trademark of sigma-aldrich Co. LLC
基本信息
MDL编号 | MFCD00217820 |
NACRES | NA.75 |
产品性质
质量水平 | 500 |
形式 | powder |
technique(s) | cell culture | hybridoma: suitable |
杂质 | endotoxin |
web metadata keyword.default | classic media, classical media, FBS, lymphoblastoid cells, cell fusion, hybrid cells |
组分 | NaHCO3: no glucose: 4.5 g/L (Dextro) L-glutamine: 0.3 g/L HEPES: 3.5745 g/L phenol red: 0.0053 g/L |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich