CRISPR &MISSION^® Lentiviral Packaging Mix

产品说明

一般描述

The CRISPR & MISSION Lentiviral Packaging Mix is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene.
Lentiviral particles are generated from three main components:

• The packaging vector, which contains the minimal set of lentiviral genes required to generate the virion structural proteins and packaging functions.
• The vesicular stomatitis virus G-protein envelope vector, which provides the heterologous envelope for pseudotyping and allows these lentiviral particles to efficiently deliver the transfer sequence of interest to a wide variety of cell types, including primary and non-dividing cells
• The lentiviral transfer vector, which contains the sequence of interest as well as the cis acting sequences necessary for packaging.

The Lentiviral Packaging Mix contains the first two components; it is designed to be co-transfected along with a compatible lentiviral transfer vector in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications. This product is recommended for packaging Lenti CRISPR, Lenti ORF, and Lenti shRNA vectors.

应用

功能性基因组学研究/筛选/靶标验证

法律信息

CRISPR Use License Agreement
MISSION is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRES

NA.51

产品性质

质量水平	200
浓度	100 ng/μL (in Tris-EDTA)
application(s)	CRISPR
运输	dry ice
储存温度	−20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable