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Cleland′s Reagent, ULTROL® Grade

品牌
Millipore
CAS
3483-12-3
货号
233153-M
规格纯度
A protective agent for SH groups Absorbance: (100 mM, 280 nm):≤0.05. Oxidized dithiothreitol:≤0.5%.
参考价格
1160.45 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
3483-12-3
Cleland′s Reagent, ULTROL® Grade
Dithiothreitol, DTT
产品介绍:

产品说明

一般描述

A protective agent for SH groups.
Absorbance: (100 mM, 280 nm):≤0.1. Oxidized dithiothreitol:≤0.5%.
DTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes, protecting protein sulfhydryls and restoring enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL Reagent, Cat. No. 233157) can be used. Absorbance: (100 mM, 280 nm):≤0.05. Oxidized dithiothreitol:≤0.5%.

警告

Toxicity: Harmful (C)

重悬

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Avoid contact with metals. Stock solutions are stable for up to 3 months at -20°C.

其他说明

Chau, M-H., and Nelson, J.W. 1991. FEBS Lett.291, 296.
Lauriault, V.V.M., and O′Brien, P.J. 1991. Mol. Pharmacol.40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett.42, 199.
Zhang, R., and Snyder, G.H. 1988. Biochemistry27, 3785.
Shaked, Z., et al. 1980. Biochemistry19, 4156.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem.243, 716.
Cleland, W.W. 1964. Biochemistry3, 480.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式C4H10O2S2
分子量154.25
MDL编号MFCD00004877

产品性质

质量水平100
测定≥99% (titration)
形式solid
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
颜色 white
溶解性water: 200 mM
ethanol: soluble
痕量阳离子heavy metals: <1 ppm
储存温度2-8℃
InChI1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
InChI keyVHJLVAABSRFDPM-IMJSIDKUSA-N

安全信息

象形图GHS05GHS07
警示用语:Danger
危险声明H302 - H315 - H318
预防措施声明P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
危险分类Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
储存分类代码11 - Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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