产品说明
一般描述
A protective agent for SH groups.
Absorbance: (100 mM, 280 nm):≤0.1. Oxidized dithiothreitol:≤0.5%.
DTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes, protecting protein sulfhydryls and restoring enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL
警告
Toxicity: Harmful (C)
重悬
Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Avoid contact with metals. Stock solutions are stable for up to 3 months at -20°C.
其他说明
Chau, M-H., and Nelson, J.W. 1991. FEBS Lett.291, 296.
Lauriault, V.V.M., and O′Brien, P.J. 1991. Mol. Pharmacol.40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett.42, 199.
Zhang, R., and Snyder, G.H. 1988. Biochemistry27, 3785.
Shaked, Z., et al. 1980. Biochemistry19, 4156.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem.243, 716.
Cleland, W.W. 1964. Biochemistry3, 480.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C4H10O2S2 |
| 分子量 | 154.25 |
| MDL编号 | MFCD00004877 |
产品性质
| 质量水平 | 100 |
| 测定 | ≥99% (titration) |
| 形式 | solid |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze |
| 颜色 | white |
| 溶解性 | water: 200 mM ethanol: soluble |
| 痕量阳离子 | heavy metals: <1 ppm |
| 储存温度 | 2-8℃ |
| InChI | 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1 |
| InChI key | VHJLVAABSRFDPM-IMJSIDKUSA-N |
安全信息
| 象形图 |   |
| 警示用语： | Danger |
| 危险声明 | H302 - H315 - H318 |
| 预防措施声明 | P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 |
| 危险分类 | Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
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