产品说明
细胞系来源
Hamster Syrian kidney
细胞系描述
Derived from the parent line BHK-21 (Clone-13) (Sigma Catalogue number. 85011433). The cells have been adapted to growth in suspension and have the same virus susceptibility as the parent line.
应用
Virus replication studies
培养基
GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).
传代培养常规
Maintain cultures between 2-9x100,000 cells/ml (split ratio of 1:3 to 1:6); 5% CO2; 37℃. Cells need to be grown in a techni-stirrer or they may start to re-attach.
其他说明
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产品性质
生物来源 | hamster kidney |
生长模式 | Suspension |
核型 | 2n = 44, pseudodiploid |
形态学 | Not specified |
产品 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
储存温度 | −196℃ |
Sigma-Aldrich