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CHO Protein-Free Cell Line from Chinese hamster ovary cells

货号
CB_00102307
规格纯度
00102307
参考价格
4633 *本价格含增值税费
促销
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数量
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产品名称:
CHO Protein-Free Cell Line from Chinese hamster ovary cells
产品介绍:

产品说明

细胞系来源

Chinese hamster ovary

细胞系描述

A subclone of the parental CHO line originated by Puck in 1957. The cells have an absolute requirement for L-proline. The cells have been adapted for growth in Sigma CHO Protein Free Medium (C5467). They have been cryopreserved in Sigma Cell Freezing Medium - Serum Free (C2639).

培养基

Sigma CHO-protein free medium + 4mM Glutamine. Cells should be frozen in either Sigma Cell Freezing Medium - Serum Free (C2639) with 10% DMSO or Sigma CHO Protein Free Medium (C5467) with 7.5%-10% DMSO

传代培养常规

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37℃ for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 105 cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO2; 37℃.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

产品性质

生物来源hamster ovary
描述Chinese hamster ovary
生长模式Suspension
核型Not specified
形态学Suspension
产品Not specified
受体Not specified
technique(s)cell culture | mammalian: suitable
运输dry ice
储存温度−196℃

Sigma-Aldrich

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