M-1

产品说明

细胞系来源

Mouse kidney cortical collecting duct, SV40 transformed

细胞系描述

Derived from the cortical collecting duct (CCD) of a mouse, transgenic for the early region of SV40 (Tg(SV40E)Bri/7). They express many characteristics of the CCD like epithelial morphology and CCD-specific antigens. M-1 cells grown on permeable supports exhibited a high transepithelial resistance concomitant with the development of a lumen-negative transepithelial potential difference. The M-1 cell line and sub-clones exhibit principal cell (PC) functions and ß-intercalated cell (ß-ICC) functions, both typical for the heterogeneous epithelium found in the renal collecting duct. M-1 cells and sub-clones show a heterogeneous expression of PC and ß-ICC antigens with 5-10% exhibiting a dual PC/ß-ICC phenotype.

应用

Kidney cell studies, differentiation studies.

培养基

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 5μm Dexamethasone (DXMT) + 5% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃. Prolonged incubation with trypsin may be necessary for subculture.

其他说明

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产品性质