NCTC clone 1469

产品说明

细胞系来源

Mouse C3H/An liver

细胞系描述

Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).

应用

Cell suspension systems. Biochemical, nutritional and virus studies. VSV(Indiana)

培养基

NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10CO₂; 37℃. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearan

其他说明

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产品性质