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NCTC clone 929

货号
CB_88102702
规格纯度
88102702
参考价格
4633 *本价格含增值税费
促销
服务
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数量
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产品名称:
NCTC clone 929
产品介绍:

产品说明

细胞系来源

Mouse C3H/An connective tissue

细胞系描述

From parental strain L which was derived from normal subcutaneous areolar and adipose tissue of 100 day old male C3H/An. Used in development of replicate culture techniques, chemically defined media, fluid suspension culture methods and virus assays. Susceptible to pseudorabies virus and vesicular stomatitis (Indiana strain) virus.

应用

Replication, biochemical, neoplastic transformation, nutritional, metabolic enzyme, morphological, chromosome, immunologic, radiation, toxicologic and virus studies

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃. Freeze in 10% glycerol/90% FBS. After resuscitation cells may take up to 4 days to establish growth.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

产品性质

生物来源mouse connective tissue
生长模式Adherent
核型2n = 40
形态学Fibroblast
产品Not specified
受体Not specified
technique(s)cell culture | mammalian: suitable
运输dry ice
储存温度−196℃

Sigma-Aldrich

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