产品说明
细胞系来源
Rat Sprague-Dawley lung
细胞系描述
Derived from the lung tissue of a normal germ-free, 18 day gestation, Sprague-Dawley rat foetus. At least 45 population doublings expected.
培养基
Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
产品性质
| 生物来源 | rat lung |
| 生长模式 | Adherent |
| 核型 | 2n = 42 |
| 形态学 | Fibroblast |
| 产品 | Not specified |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
| 储存温度 | −196℃ |
Sigma-Aldrich
m.cnreagent.com
