产品说明
细胞系来源
Rat Fischer fibroblast
细胞系描述
Derived from a subclone of a BUdR-resistant strain of a Fischer rat fibroblast 3T3-like line RAT-1. The line is suitable for transformation and TGF (SGF) assays as an indicator cell line.
应用
Transformation studies
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Vitamins + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
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产品性质
生物来源 | rat embryo |
生长模式 | Adherent |
核型 | Not specified |
形态学 | Fibroblast |
产品 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
储存温度 | −196℃ |