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208F

货号
CB_85103116
规格纯度
85103116
参考价格
4633 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
208F
产品介绍:

产品说明

细胞系来源

Rat Fischer fibroblast

细胞系描述

Derived from a subclone of a BUdR-resistant strain of a Fischer rat fibroblast 3T3-like line RAT-1. The line is suitable for transformation and TGF (SGF) assays as an indicator cell line.

应用

Transformation studies

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Vitamins + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

产品性质

生物来源rat embryo
生长模式Adherent
核型Not specified
形态学Fibroblast
产品Not specified
受体Not specified
technique(s)cell culture | mammalian: suitable
运输dry ice
储存温度−196℃

Sigma-Aldrich

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