EBTr

产品说明

细胞系来源

Bovine foetal trachea

细胞系描述

Derived from the trachea of a male bovine foetus. The cell line can be used for isolation, replication and quantitation of BVDV, IBRV and parainfluenza type 3 virus. Growth and morphological characteristics decline beyond passage number 55.

应用

Virus studies: BVDV, IBRV and parainfluenza type 3 virus.

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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产品性质