产品说明
细胞系来源
American Opossum, kidney
细胞系描述
Derived from proximal tubule of a kidney taken from an adult female opossum. Cells retain proximal tubule function. Possesses the following receptors: α 2 - adrenergic, insulin, serotonin, parathyroid hormone and atrial natriuretic factor.
应用
Kidney function studies
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
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产品性质
生物来源 | opossum kidney |
生长模式 | Adherent |
核型 | Not specified |
形态学 | Not specified |
产品 | Not specified |
受体 | Insulin, serotonin, alpha 2-adrenergic parathy. Hormone |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
储存温度 | −196℃ |
Sigma-Aldrich