产品说明
细胞系来源
Mouse BALB/3T3 clone A31
细胞系描述
Derived by SV40 transformation of the line BALB/3T3 clone A31. The cells exhibit a high saturation density, lack contact inhibition and form tumours in appropriate hosts.
应用
Transformation, tumourigenicity and virus studies: SV40
培养基
DMEM + 2mM Glutamine + 10% New Born Calf Serum (NBCS) or (5% NBCS + 5% Foetal Bovine Serum (FBS)).
传代培养常规
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1x1,000 to 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
产品性质
生物来源 | mouse embryo |
生长模式 | Adherent |
核型 | 2n = 40, modal No 41 |
形态学 | Fibroblast |
产品 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
储存温度 | −196℃ |
Sigma-Aldrich