产品说明
一般描述
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice .
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
特异性
Binds all mouse Igs. Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fab fragment. The product does not react with mouse IgG, Fc fragment, or human IgG.
免疫原
Purified mouse IgG Fab fragment
应用
Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm.
Anti-Mouse IgG (Fab specific)−Alkaline Phosphatase antibody produced in goat has been used in:
生化/生理作用
Immunoglobulin G (IgG) helps in opsonization and antibody dependent cell mediated cytotoxicity. It mainly helps in immune defense.
其他说明
Antibody adsorbed with human IgG.
外形
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide
制备说明
Adsorbed to reduce background staining with human samples.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD00162620 |
| NACRES | NA.46 |
产品性质
| 质量水平 | 200 |
| 生物来源 | goat |
| 重组 | expressed in goat |
| 偶联物 | alkaline phosphatase conjugate |
| 抗体形式 | affinity isolated antibody |
| antibody product type | secondary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| species reactivity | mouse |
| should not react with | human |
| technique(s) | direct ELISA: 1:40,000 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 western blot (chemiluminescent): 1:30,000 |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
