产品说明
一般描述
Sigma′s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
应用
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
组分
10mM Tris-HCl 中的溶液,pH 8.0,1mM EDTA
其他说明
Contains 22 fragments, 8–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5.
制备说明
Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.
基本信息
NACRES | NA.25 |
产品性质
质量水平 | 100 |
形式 | liquid |
用途 | 1000 uses (0.5 mg size) 200 uses (0.1 mg size) |
适用性 | suitable for electrophoresis (DNA) |
储存温度 | −20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich