产品说明

一般描述

SOLVO Biotechnology offers ready-to-use assay kits for efflux and uptake transporters. SOLVO′s PREDEASY^™ ATPase Assay Kit provides everything necessary to conduct ATPase activation and inhibition assays to better understand the nature of the interaction between a compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated.

应用

SOLVO′s PREDEASY^™ ATPase Assay Kit is a unique product for measuring the ATPase-dependent drug - ABC membrane transporter interaction in a convenient, "ready-to-assay" package. The ATPase assay is an in vitro membrane assay designed to indicate the nature of the interaction between the compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated using membranes from baculovirus-infected insect cells or mammalian cell membranes containing high levels of human or rodent wild-type transporters. ABC transporters mediate the transport of substrates against a concentration gradient using energy derived from ATP hydrolysis, which is proportional to the transporter activity and could easily be detected with a colorimetric method.
PREDIVEZ^™ PREDEASY^™ Kits contain all reagents (chemicals) and solutions necessary to conduct ATPase activation and inhibition assays. You only have to add water. Each kit comes with a CD that includes the assay protocol, data sheet, SDS, electronic data processing, evaluation templates and the reference data. The recommended layout allows activation and inhibition measurements in duplicates for 8 concentrations of the test article on one 96-well plate with all of the relevant controls.
PREDEASY^™ ATPase Assay Kits are available for 3 and 10 plates for the transporters recommended by the FDA and the EMA.

外形

Supplied as frozen membrane vesicles, containing 5 mg/ml membrane protein, labeled with volume, catalog number (transporter), date of production, protocols and necessary reagents sufficient for the analysis of 3 or 10, 96-well plates.

法律信息

Distributed for SOLVO Biotechnology, Inc.
PREDEASY is a trademark of Solvo Biotechnology
PREDIVEZ is a trademark of Solvo Biotechnology

产品性质

安全信息

象形图
警示用语：	Danger
危险声明	H225 - H290 - H314 - H335 - H350 - H412
预防措施声明	P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
危险分类	Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Skin Corr. 1B - STOT SE 3
靶器官	Respiratory system
储存分类代码	3 - Flammable liquids
WGK	WGK 3