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Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat

品牌
Sigma-Aldrich
货号
B8895
规格纯度
affinity isolated antibody, buffered aqueous solution
参考价格
1402.31 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
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产品名称:
Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat
产品介绍:

产品说明

一般描述

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule)-Biotin antibody reacts with rabbit serum and rabbit IgG. The product does not cross-react with human IgG.
IgG antibodies regulate several functions such as phagocytosis and complement activation. Thus they play a crucial role in facilitating cytological immune responses. Anti-rabbit IgG (whole molecule)–biotin antibody can be used in immunohistochemistry. The product shows minimal interspecies cross reactivity to human IgG hence can be used in enzyme immunoassays or dot blot in presence of human IgG. Anti-rabbit IgG (whole molecule)–biotin antibody reacts specifically to all rabbit immunoglobulin but has no reactivity with human IgG.

特异性

Binds all rabbit Igs.

免疫原

Purified rabbit IgG

应用

Anti-Rabbit IgG (whole molecule)-Biotin antibody has been used in

  • Immunofluorescence
  • Immunohistochemistry
  • Western blot
  • Direct enzyme linked immunosorbent assay (ELISA)

生化/生理作用

Avidin, Streptavidin or ExtrAvidine show strong affinity interaction with biotin rendering the biotin-avidin system an extremely effective tool in molecular biology, protein chemistry and immunology. The high specificity of the biotinylated antibody to rabbit IgG and the stability of the biotin-avidin complex has been exploited in variety of reagents (Avidin, Streptavidin or ExtrAvidin conjugated to FITC, TRITC, Peroxidase or Alkaline Phosphatase), for the detection and quantitation of rabbit IgG.

其他说明

Antibody adsorbed with human IgG.

外形

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide

制备说明

Adsorbed to reduce background staining with human samples.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00162783
NACRESNA.46

产品性质

质量水平200
生物来源goat
偶联物biotin conjugate
抗体形式affinity isolated antibody
antibody product typesecondary antibodies
克隆polyclonal
形式buffered aqueous solution
technique(s)direct ELISA: 1:200,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800
western blot: 1:400,000-1:800,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)
储存温度−20℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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