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L-Glutamic Dehydrogenase (NADP) from Proteus sp.

品牌
Sigma-Aldrich
CAS
9029-11-2
货号
G4387
规格纯度
buffered aqueous solution, ≥4,000 units/mL
参考价格
2342.64 *本价格含增值税费
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数量
-+
产品名称:
9029-11-2
L-Glutamic Dehydrogenase (NADP) from Proteus sp.
L-Glutamate:NADP+ oxidoreductase (deaminating)
产品介绍:

产品说明

应用

This enzyme is useful for enzymatic determination of NH3, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease (URH-201) in clinical analysis. In vitro, various activity assays of this enzyme examine the conversion of α-ketoglutarate to L-glutamate, in the presence of excess ammonium ions (NH4+) and NADPH.

生化/生理作用

L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.

物理属性

Isoelectric point : 4.6
Michaelis constants : 1.1 X 10-3M (NH3), 3.4 X 10-4M (α-Ketoglutarate)
1.2 X 10-3M (L-Glutamate), 1.4 X 10-5M (NADPH), 1.5 X 10-5M (NADP+)
Structure : 6 subunits (M.W.50,000) per mol of enzyme
Inhibitors : Hg++, Cd++, p-chloromercuribenzoate, pyridine, 4-4′-dithiopyridine,
2,2′-dithiopyridine
Optimum pH : 8.5 (α-KG→L-Glu) 9.8 (L-Glu→α-KG)
Optimum temperature : 45oC(α-KG−L-Glu) 45-55oC (L-Glu→α-KG)
pH stability : pH 6.0 - 8.5 (25oC, 20hr)
Thermal stability : below 50oC (pH 7.4, 10min)

单位定义

One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 8.3 at 30 ℃ in the presence of ammonium ions and NADPH.

外形

Solution in 50 mM Tris HCl, pH 7.8, 5 mM Na2EDTA containing 0.05% sodium azide

其他说明

Note: Do not confuse with non-specific L-GLDH, EC 1.4.1.3.

基本信息

酶学委员会(EC)编号1.4.1.4 ( BRENDA | IUBMB )
MDL编号MFCD00131461
NACRESNA.54

产品性质

质量水平200
生物来源bacterial (Proteus spp.)
形式buffered aqueous solution
specific activity≥4,000 units/mL
分子量 ~300 kDa
储存温度2-8℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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