产品说明
应用
This enzyme is useful for enzymatic determination of NH3, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease (URH-201) in clinical analysis. In vitro, various activity assays of this enzyme examine the conversion of α-ketoglutarate to
生化/生理作用
L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.
物理属性
Isoelectric point : 4.6
Michaelis constants : 1.1 X 10-3M (NH3), 3.4 X 10-4M (α-Ketoglutarate)
1.2 X 10-3M (L-Glutamate), 1.4 X 10-5M (NADPH), 1.5 X 10-5M (NADP+)
Structure : 6 subunits (M.W.50,000) per mol of enzyme
Inhibitors : Hg++, Cd++, p-chloromercuribenzoate, pyridine, 4-4′-dithiopyridine,
2,2′-dithiopyridine
Optimum pH : 8.5 (α-KG→L-Glu) 9.8 (L-Glu→α-KG)
Optimum temperature : 45oC(α-KG−L-Glu) 45-55oC (L-Glu→α-KG)
pH stability : pH 6.0 - 8.5 (25oC, 20hr)
Thermal stability : below 50oC (pH 7.4, 10min)
单位定义
One unit will reduce 1.0 μmole of α-ketoglutarate to
外形
Solution in 50 mM Tris HCl, pH 7.8, 5 mM Na2EDTA containing 0.05% sodium azide
其他说明
Note: Do not confuse with non-specific
基本信息
酶学委员会(EC)编号 | 1.4.1.4 ( BRENDA | IUBMB ) |
MDL编号 | MFCD00131461 |
NACRES | NA.54 |
产品性质
质量水平 | 200 |
生物来源 | bacterial (Proteus spp.) |
形式 | buffered aqueous solution |
specific activity | ≥4,000 units/mL |
分子量 | ~300 kDa |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich