293 N3S

产品说明

细胞系来源

Human embryo kidney

细胞系描述

Adapted for growth in suspension by passaging 293 cells in nude mice. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions.

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 12,14
D16S539: 9
D5S818: 9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19

培养基

Joklik′s MEM + 2mM Glutamine + 10% Horse Serum.

传代培养常规

Maintain cultures between 5 x 10,000 to 5 x 100,000 cells/ml in a Techne stirrer; 5% CO₂; 37℃. Cell doubling time is 30 hours.

其他说明

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产品性质